Indian Journal of Community Medicine

ORIGINAL ARTICLE
Year
: 2014  |  Volume : 39  |  Issue : 3  |  Page : 156--160

Antibiotic susceptibility profile of bacteria isolated from natural sources of water from rural areas of East Sikkim


Shubra Poonia, T Shantikumar Singh, Dechen C Tsering 
 Department of Microbiology, Sikkim Manipal Institute of Medical Sciences, Sikkim Manipal University, Gangtok, Sikkim, India

Correspondence Address:
Dr. T Shantikumar Singh
Department of Microbiology, Sikkim Manipal Institute of Medical Sciences, Sikkim Manipal University, Gangtok, Sikkim
India

Background: Contamination of water, food, and environment with antibiotic-resistant bacteria poses a serious public health issue. Objective: The objective was to study the bacterial pollution of the natural sources of water in east Sikkim and to determine the antimicrobial profile of the bacterial isolates. Materials and Methods: A total of 225 samples, 75 each during winter, summer, and monsoon season were collected from the same source in every season for bacteriological analysis by membrane filtration method. Antibiotic susceptibility test was performed using standard disc diffusion method. Results: A total of 19 bacterial species of the genera Escherichia, Klebsiella, Proteus, Salmonella, Shigella, Enterobacter, Citrobacter, Morganella, Pseudomonas, Acinetobacter, Flavobacterium, and Serratia were isolated and their antimicrobial sensitivity tested. Generally, most bacterial isolates except Salmonella and Shigella species were found resistant to commonly used antibiotics such as ampicillin (57.5%), trimethoprim/sulfamethoxaole (39.1%), amoxicillin/clavulanic acid (37.4%), cefixime (34.5%), tetracycline (29.1%), ceftazidime (26.3%), ofloxacin (25.9%), amikacin (8.7%), and gentamicin (2.7%) but sensitive to imipenem and piperacillin/tazobactam. Conclusion: Natural sources of water in east Sikkim are grossly contaminated with bacteria including enteropathogens. The consumption of untreated water from these sources might pose health risk to consumers.


How to cite this article:
Poonia S, Singh T S, Tsering DC. Antibiotic susceptibility profile of bacteria isolated from natural sources of water from rural areas of East Sikkim.Indian J Community Med 2014;39:156-160


How to cite this URL:
Poonia S, Singh T S, Tsering DC. Antibiotic susceptibility profile of bacteria isolated from natural sources of water from rural areas of East Sikkim. Indian J Community Med [serial online] 2014 [cited 2022 May 27 ];39:156-160
Available from: https://www.ijcm.org.in/article.asp?issn=0970-0218;year=2014;volume=39;issue=3;spage=156;epage=160;aulast=Poonia;type=0